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Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
10.00 | 3.106 | 3.055 |
5.00 | 2.024 | 1.973 |
2.50 | 1.179 | 1.129 |
1.25 | 0.691 | 0.641 |
0.63 | 0.372 | 0.322 |
0.32 | 0.217 | 0.167 |
0.16 | 0.144 | 0.094 |
0.00 | 0.050 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 92-110% | 101% |
EDTA plasma(n=5) | 88-105% | 95% |
Heparin plasma(n=5) | 90-102% | 98% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 93-105% | 85-102% | 91-110% | 91-108% |
EDTA plasma(n=5) | 90-104% | 90-106% | 88-108% | 92-109% |
Heparin plasma(n=5) | 88-103% | 94-108% | 95-105% | 88-107% |
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