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標準曲線
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 5000.00 | 2.781 | 2.716 |
| 2500.00 | 1.701 | 1.637 |
| 1250.00 | 0.906 | 0.842 |
| 625.00 | 0.508 | 0.443 |
| 312.50 | 0.319 | 0.254 |
| 156.25 | 0.177 | 0.113 |
| 78.13 | 0.120 | 0.056 |
| 0.00 | 0.064 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 88-99% | 94% |
| EDTA plasma(n=5) | 102-110% | 104% |
| Heparin plasma(n=5) | 98-104% | 101% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 93-106% | 88-102% | 85-101% | 96-107% |
| EDTA plasma(n=5) | 90-103% | 94-108% | 90-106% | 98-105% |
| Heparin plasma(n=5) | 91-100% | 98-107% | 92-108% | 93-102% |
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