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下載說明 ①Overview 文獻
日期：2024-12-09
IF：5.3標準曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 2.035 | 1.928 |
| 5.00 | 1.593 | 1.486 |
| 2.50 | 1.183 | 1.076 |
| 1.25 | 0.752 | 0.645 |
| 0.63 | 0.546 | 0.439 |
| 0.32 | 0.354 | 0.247 |
| 0.16 | 0.232 | 0.125 |
| 0.00 | 0.107 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant IFABP/FABP2 and the recovery rates were calculated by comparing the measured value to the expected amount of IFABP/FABP2 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 82-95% | 88% |
| EDTA plasma(n=5) | 80-95% | 87% |
| Heparin plasma(n=5) | 86-99% | 92% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFABP/FABP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-98% | 92-105% | 95-107% | 82-96% |
| EDTA plasma(n=5) | 81-94% | 93-107% | 93-102% | 91-105% |
| Heparin plasma(n=5) | 89-98% | 81-94% | 96-105% | 92-103% |
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