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            大鼠P物質(zhì)(SP)酶聯(lián)免疫吸附檢測(cè)試劑盒
            ELK1510
            規(guī)格: 價(jià)格:
            48T ¥1960.00
            96T ¥2800.00

            Overview 文獻(xiàn)

            Product name: Rat SP(Substance P) ELISA Kit
            Reactivity: Rat
            Alternative Names: Substance P
            Assay Type: Sandwich
            Sensitivity: 4.72 pg/mL
            Standard: 1000 pg/mL
            Detection Range: 15.63-1000 pg/mL
            Sample Type: Serum, plasma and other biological fluids
            Assay Length: 3.5h
            Research Area: Endocrinology;Neuro science;Hormone metabolism;
            Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SP in the samples is then determined by comparing the OD of the samples to the standard curve.

            標(biāo)準(zhǔn)曲線

            Concentration (pg/mL) OD Corrected OD
            1000.00 1.964 1.869
            500.00 1.612 1.517
            250.00 1.176 1.081
            125.00 0.943 0.848
            62.50 0.621 0.526
            31.25 0.354 0.259
            15.63 0.232 0.137
            0.00 0.095 0.000

            精密度

            Intra-assay Precision (Precision within an assay):CV%<8%

            Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

            Inter-assay Precision (Precision between assays):CV%<10%

            Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

            回收率

            Matrices listed below were spiked with certain level of recombinant SP and the recovery rates were calculated by comparing the measured value to the expected amount of SP in samples.
            Matrix Recovery range Average
            serum(n=5) 88-103% 95%
            EDTA plasma(n=5) 77-95% 86%
            Heparin plasma(n=5) 92-106% 99%

            線性

            The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
            Matrix 1:2 1:4 1:8 1:16
            serum(n=5) 79-95% 96-105% 85-96% 90-99%
            EDTA plasma(n=5) 83-97% 81-103% 89-101% 81-98%
            Heparin plasma(n=5) 85-101% 93-104% 87-103% 91-102%
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