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            人Ⅲ型膠原交聯(lián)羧基端肽(CTXIII)酶聯(lián)免疫吸附檢測試劑盒
            ELK1543
            規(guī)格: 價格:
            48T ¥1960.00
            96T ¥2800.00

            Overview

            Product name: Human CTXIII(Cross Linked C-Telopeptide Of Type III Collagen) ELISA Kit
            Reactivity: Human
            Alternative Names: CTX-III; CTX3
            Assay Type: Competitive Inhibition
            Sensitivity: 47.8 pg/mL
            Standard: 10000 pg/mL
            Detection Range: 156.25-10000 pg/mL
            Sample Type: Serum, plasma and other biological fluids
            Assay Length: 2h
            Research Area: Metabolic pathway;Nutrition metabolism;Bone metabolism;
            Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CTXIII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTXIII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTXIII in the samples is then determined by comparing the OD of the samples to the standard curve.

            標(biāo)準(zhǔn)曲線

            Concentration (pg/mL) OD Corrected OD
            10000.00 0.189
            5000.00 0.386
            2500.00 0.575
            1250.00 0.823
            625.00 1.112
            312.50 1.537
            156.25 1.775
            0.00 2.226

            精密度

            Intra-assay Precision (Precision within an assay):CV%<8%

            Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

            Inter-assay Precision (Precision between assays):CV%<10%

            Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

            回收率

            Matrices listed below were spiked with certain level of recombinant CTXIII and the recovery rates were calculated by comparing the measured value to the expected amount of CTXIII in samples.
            Matrix Recovery range Average
            serum(n=5) 84-99% 91%
            EDTA plasma(n=5) 79-91% 85%
            Heparin plasma(n=5) 81-97% 89%

            線性

            The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CTXIII and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
            Matrix 1:2 1:4 1:8 1:16
            serum(n=5) 87-95% 85-98% 82-97% 92-101%
            EDTA plasma(n=5) 83-92% 91-96% 85-93% 95-101%
            Heparin plasma(n=5) 81-94% 96-105% 92-103% 82-96%
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