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            人β-骨膠原交聯(lián)(bCTx)酶聯(lián)免疫吸附檢測試劑盒
            ELK1544
            規(guī)格: 價(jià)格:
            48T ¥1960.00
            96T ¥2800.00

            Overview

            Product name: Human bCTx(Beta-Crosslaps) ELISA Kit
            Reactivity: Human
            Alternative Names: Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction
            Assay Type: Competitive Inhibition
            Sensitivity: 36.16 pg/mL
            Standard: 8000 pg/mL
            Detection Range: 125-8000 pg/mL
            Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
            Assay Length: 2h
            Research Area: Bone metabolism;
            Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human bCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.

            標(biāo)準(zhǔn)曲線

            Concentration (pg/mL) OD Corrected OD
            8000.00 0.199
            4000.00 0.397
            2000.00 0.596
            1000.00 0.812
            500.00 1.157
            250.00 1.592
            125.00 1.789
            0.00 2.211

            精密度

            Intra-assay Precision (Precision within an assay):CV%<8%

            Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

            Inter-assay Precision (Precision between assays):CV%<10%

            Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

            回收率

            Matrices listed below were spiked with certain level of recombinant bCTx and the recovery rates were calculated by comparing the measured value to the expected amount of bCTx in samples.
            Matrix Recovery range Average
            serum(n=5) 87-99% 93%
            EDTA plasma(n=5) 78-90% 84%
            Heparin plasma(n=5) 89-107% 98%

            線性

            The linearity of the kit was assayed by testing samples spiked with appropriate concentration of bCTx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
            Matrix 1:2 1:4 1:8 1:16
            serum(n=5) 82-94% 83-96% 91-103% 82-99%
            EDTA plasma(n=5) 78-92% 85-96% 93-102% 95-104%
            Heparin plasma(n=5) 88-104% 84-98% 95-105% 88-102%
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