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            小鼠β-骨膠原交聯(lián)(bCTx)酶聯(lián)免疫吸附檢測(cè)試劑盒
            ELK1545
            規(guī)格: 價(jià)格:
            48T ¥1960.00
            96T ¥2800.00

            Overview

            Product name: Mouse bCTx(Beta-Crosslaps) ELISA Kit
            Reactivity: Mouse
            Alternative Names: Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction
            Assay Type: Competitive Inhibition
            Sensitivity: 27.5 pg/mL
            Standard: 5000 pg/mL
            Detection Range: 78.13-5000 pg/mL
            Sample Type: Serum, plasma, urine and other biological fluids
            Assay Length: 2h
            Research Area: Bone metabolism;
            Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse bCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.

            標(biāo)準(zhǔn)曲線(xiàn)

            Concentration (pg/mL) OD Corrected OD
            5000.00 0.191
            2500.00 0.396
            1250.00 0.611
            625.00 0.833
            312.50 1.197
            156.25 1.583
            78.13 1.762
            0.00 2.123

            精密度

            Intra-assay Precision (Precision within an assay):CV%<8%

            Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

            Inter-assay Precision (Precision between assays):CV%<10%

            Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

            回收率

            Matrices listed below were spiked with certain level of recombinant bCTx and the recovery rates were calculated by comparing the measured value to the expected amount of bCTx in samples.
            Matrix Recovery range Average
            serum(n=5) 87-99% 93%
            EDTA plasma(n=5) 80-95% 87%
            Heparin plasma(n=5) 90-107% 98%

            線(xiàn)性

            The linearity of the kit was assayed by testing samples spiked with appropriate concentration of bCTx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
            Matrix 1:2 1:4 1:8 1:16
            serum(n=5) 92-104% 93-99% 87-953% 82-93%
            EDTA plasma(n=5) 88-96% 90-101% 93-102% 85-94%
            Heparin plasma(n=5) 79-91% 89-103% 82-95% 86-99%
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