久久久国产99久，在线观看无码AV黄色，无码人妻一区二区精品视频，国产三区四区在线视频，一区二区三区四区无码，国产一级婬片永久免费看，欧美国产三级黄片，人妻忍着娇喘被中进中出视频

產(chǎn)品中心

人蛋白激酶Bγ(PKBg)酶聯(lián)免疫吸附檢測試劑盒

ELK1550

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human PKBg(Protein Kinase B Gamma) ELISA Kit
Reactivity:	Human
Alternative Names:	PKBG; PRKB-G; AKT3; RAC-PK-Gamma; RAC-Gamma; STK2; RAC-Gamma Serine/Threonine-Protein Kinase; V-Akt Murine Thymoma Viral Oncogene Homolog 3
Assay Type:	Sandwich
Sensitivity:	0.6 ng/mL
Standard:	100 ng/mL
Detection Range:	1.57-100 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Tumor immunity;Endocrinology;Neuro science;
Uniprot ID:	Q9Y243
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PKBg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PKBg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PKBg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PKBg in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
100.00	2.231	2.150
50.00	1.636	1.555
25.00	1.115	1.034
12.50	0.857	0.776
6.25	0.527	0.446
3.13	0.335	0.254
1.57	0.226	0.145
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PKBg and the recovery rates were calculated by comparing the measured value to the expected amount of PKBg in samples.

Matrix	Recovery range	Average
serum(n=5)	78-90%	84%
EDTA plasma(n=5)	85-99%	92%
Heparin plasma(n=5)	78-92%	85%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKBg and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.