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日期：2024-10-02
IF：3標(biāo)準(zhǔn)曲線
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 1.933 | 1.840 |
| 5.00 | 1.697 | 1.604 |
| 2.50 | 1.037 | 0.944 |
| 1.25 | 0.838 | 0.745 |
| 0.63 | 0.509 | 0.416 |
| 0.32 | 0.318 | 0.225 |
| 0.16 | 0.187 | 0.094 |
| 0.00 | 0.093 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant CK-18 and the recovery rates were calculated by comparing the measured value to the expected amount of CK-18 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 80-95% | 87% |
| EDTA plasma(n=5) | 78-92% | 85% |
| Heparin plasma(n=5) | 78-96% | 87% |
線性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CK-18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 92-104% | 82-96% | 95-102% | 87-98% |
| EDTA plasma(n=5) | 86-93% | 91-105% | 87-98% | 89-94% |
| Heparin plasma(n=5) | 88-104% | 84-98% | 95-105% | 88-102% |
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